The samples were tested for SAFV by using real-time RT-PCR primer/probe, and all positive samples were genotyped by partial sequencing of the viral protein (VP) 1 gene (6).
To determine the evolutionary history of strains of SAFV identified in persons in Denmark, we combined the VP1 sequences collected here with all others available on GenBank.
We tested previously submitted CSF samples for SAFV, reviewed the patients' medical records, and sequenced the viruses isolated.
We selected fecal samples from 479 children [less than or equal to] 5 years of age with gastroenteritis that had been submitted for viral diagnostic testing from September 2009 through February 2010 and tested them for SAFV.
To determine the prevalence of SAFV infection among children in Malaysia, we conducted a seroprevalence study by using 400 serum samples collected during 2009 from children 10-12 years of age under the national hepatitis B postvaccination serosurvey.
In summary, a new SAFV was discovered in Malaysia by direct virus isolation during an investigation of a febrile patient.
-3 pos SAFV
-2 pos + Country ([double dagger]) ([section]) SAFV
-3 neg Netherlands 4 (14) 15 (52) 11 Netherlands 20 (77) 25 (96) 5 Netherlands 29 (97) 30 (100) 1 Finland 21 (70) 24 (80) 3 Cameroon 28 (97) 29 (100) 1 Indonesia 30 (100) 30 (100) 0 SAFV
-2 neg + Country SAFV
-3 pos Netherlands 0 Netherlands 4 Netherlands 0 Finland 14 Cameroon 1 Indonesia 0 * SAFV
, Saffold virus; pos, positive; neg, negative.
Nucleotide sequences of SAFV
strains described were deposited in GenBank under accession nos.
Co-infection of klassevirus with other viral agents was lower than coinfection with recently identified viruses, such as SAFV
and cosavirus (1,2).
RNA was detected in the samples by nested reverse transcription-PCR (RT-PCR) that used primers targeting the 5' untranslated region (UTR), which generated a 540-bp amplicon (9).
6%) from the 161 asymptomatic children (LZ53010) were found to be positive for SAFV
The original detection and identification of SAFV
used a combination of traditional virus isolation in cell culture and creation and characterization of a cDNA library by DNase sequence-independent single-primer amplification (15,16).