Regarding typing of chromosome cassette mec (SCCmec
), a high percent of isolates classified as CA-MRSA and HA-MRSA harbored SCCmec
type IVc (n = 73, 70.
Present study showed that all oxacillin resistant strains possessed mecA and SCCmec
type II-III in 2 isolates (18.
V) was responsible for only 20% of cases (Figure, panel D).
Diversity of SCCmec
elements in Staphylococci isolated from public washrooms.
Genotyping methods include the determination of plasmid profile, ysis of chromosomal DNA, Southern hybridization, pulsed-field gel electrophoresis (PFGE), polymerase chain reaction (PCR), SCCmec
typing and sequence-typing.
Con la aplicacion de esta aproximacion se han identificado los tipos SCCmec
del I al X, y algunas variantes epidemiologicamente pertinentes (por ejemplo, los subtipos IA y IIIA) (34-36).
Para la determinacion de los tipos de SCCmec
se disenaron iniciadores dirigidos al complejo de genes ccr (tabla i): en cuanto a la determinacion de los subtipos, se emplearon los iniciadores y las condiciones reportados por Escobar y colaboradores y por Milheirico y colaboradores (24,25).
There are five major types of SCCmec
Ten MRSA strains with SCCmec
III genotype and mecA gene were obtained and characterized from the infectious sputum samples of critically ill patients in Kunming General Hospital as previously reported (An et al.
With many commercial assays, SA strains carrying SCCmec
where the mecA gene is absent (commonly called dropout mutants ) may be incorrectly classified as MRSA.
elements have been classified into eight different types (I-VIII) and some of them are divided further into subtypes [6, 7].
This observation has been attributed to amplification of the SCCmec
target in the absence of the mecA gene (the "empty cassette" phenomenon).