Among these genes were key regulators and enzymes in adipocyte lipogenesis: Both SCD1 and ACC are regulated by the transcription factor SREBP-lc.
In the present study, the simultaneous negative regulation of SCD1, AdipoR1, AdipoR2, and ACC, which are involved in prolipogenic and adiponectin-mediated antilipogenic events, may reflect skewed adipocyte gene regulation of potential epigenetic nature.
When scientists remove SCD1
activity in lab animals, their levels of fat tissue inflammation fall sharply, and their ability to respond to insulin (insulin sensitivity) rises.
report a strong correlation between SCD mRNA levels and the 16:1n7/16:0 ratios of liver TG, FFA, CE, and PL, suggesting that the fatty acid composition of the liver can be predictive of hepatic SCD1 activity.
Many previous studies in humans have relied on total lipid extracts from whole plasma or tissues to obtain fatty acid indices and infer SCD1 activity (9-12).
For ethical reasons liver tissue samples available for clinical studies are scarce; thus we aimed to establish a blood marker representing hepatic SCD1 expression and activity.
Next, we compared the SCD1 activity indices (16:1/16:0 and 18: 1/18:0) between hepatic lipid fractions and plasma VLDL-TGs as well as hepatic SCD1 mRNA expression.
Given that the detection of genetic information on 15 SNPs in the intron, exon5 and 3'UTR regions of the SCD1 gene are significantly associated with fatty acid composition and MS (Oh et al.
Fifteen SNPs of the SCD1 gene were selected based on the reference SNP of the bovine SCD1 gene (GenBank No.
FAS, ACC1 and SCD1
, a downstream factor of SREBPlc, are enzymes for de novo fatty acid synthesis and energy homeostasis (Locke et al.
Factors that regulate the SCD1
activity or rumen fermentation conditions can alter the milk content of cis-9, trans-11 CLA.
As shown in Figure 2, genes expression of SREBP-1, ACC[alpha], SCD1
, CPT1 and HMGCR in 100 nM Kp-10 treated cells were significantly down-regulated (p<0.