Irradiation protocol and the effect of SeNPs supplementation on the WBC profile of X-ray irradiated mice
Seventy two hr after irradiation each group was divided into two SeNPs or PBS supplemented subgroups each containing 5 mice.
The blood samplings have been carried out at different intervals (0, 7 and 30 days) after SeNPs administration in non-irradiated mice and at days of 10, 20 and 30 for those mice which exposed to the different doses of X-ray radiation.
At the end of oral SeNPs supplementation in non-treated normal mice some animals from both test (received SeNPs) and control groups (received PBS solution) were scarified and different tissues were removed from their body.
Effect of SeNPs oral supplementation on the WBC profile of non-irradiated mice
As mentioned in methods and materials part, the blood samples were collected at different intervals (0, 7 and 30 days) from nonirradiated mice in both groups which received SeNPs or PBS solution (control group).
Total and differential white cells counts in normal mice which supplemented or non-supplemented by SeNPs (100 [micro]g/day) at different intervals.
According to the results of Se tissue distribution there was no considerable difference between the concentrations of Se in the collected sera or some removed organs of mice which received SeNPs or PBS solution for 30 days.
After irradiation and during the first weeks, total white blood cells decreased in all irradiated animals which received SeNPs or PBS solution (control group).
In the present study, the effect of oral administration of biogenic SeNPs on the recovery rate of WBC in BM suppressed mice exposed to X-ray radiation was investigated.
In recent years, SeNPs have been of high interest to researchers due to their excellent biological properties which are similar to selenium ion even in lower dose with lower toxicity (12).