The expression levels of 3 SIRG mRNAs and 7 OSCC salivary transcripts detected by the streamlined and standard procedures were analyzed by raw quantification cycle (Cq) values.
In addition, the water group did not show any peak in the melting-curve analysis (data not shown), no matter which SIRG primers were used, indicating there was no reagent contamination during the RT-qPCR procedures.
To evaluate the stability of saliva mRNA at room temperature without stabilizing reagent and/or nuclease inhibitor, saliva samples were stored at 25 (2) [degrees]C (laboratory ambient temperature), and the 3 SIRG mRNA expression levels were assayed by using RTqPCR at day 0 and after 1, 2, and 10 weeksofstorage.
In this study we showed that 3 SIRG mRNA expression levels remained stable in ambient temperature-stored saliva supernatant for up to 10 weeks.
Expression levels of 3 SIRG mRNAs analyzed by standard procedures and the DSTA method at day 0.