OUH602 exhibited very high levels of seedling resistance to spot blotch, NTNB, SSLB, and leaf scald, and adult plant resistance to spot blotch.
Two SSLB resistance (Rsp) QTLs were identified on chromosomes 2(2H) BIN 7 to 11 and 6(6H) BIN 10 to 14 (Table 2).
In this study, we developed an AB population with the wild barley accession OUH602 as the resistance donor and Harrington as the recurrent parent, and identified a total of seven QTL for resistances to spot blotch, NTNB, SSLB, and leaf scald.
The two SSLB resistance QTLs (Rsp-qtl-2H-7-11 and Rsp-qtl-6H-10-14) identified in this study validate the QTLs identified in the OUH602/Harrington RIL population (Yun et al.
Abbreviations: AB, advanced backcross; NTNB, net type net blotch; PCR, polymerase chain reaction; QTL, quantitative trait locus; RIL, recombinant inbred line; SSLB, Septoria speckled leaf blotch; SSR, simple sequence repeat.
The parents and progeny from the OUH602/Harrington RIL population were evaluated for resistance to spot blotch, NTNB, SSLB, and leaf scald as seedlings by artificial inoculation in the greenhouse at the University of Minnesota in St.
Leaf scald and SSLB were evaluated 23 d after inoculation by a 0-to-4 and 0-to-5 scale, respectively (Jackson and Webster 1976; Toubia-Rahme and Steffenson 2004).
OUH602 exhibited very high levels of resistance to spot blotch, NTNB, SSLB, and leaf scald.
Two QTLs conferring resistance to SSLB (Rsp loci) were consistently detected on chromosomes 2(2H) and 6(6H) (Fig.
Spontaneum accession, we mapped QTLs for resistance to spot blotch, SSLB, leaf scald, NTNB, and powdery mildew.
We identified two QTLs for SSLB resistance that mapped to locations of the barley genome not previously associated with SSLB resistance.
Except for two QTLs for SSLB resistance, all the QTLs detected in this study were located within resistance gene clusters on chromosome 1(7H), 3(3H), 4(4H), 5(1H), and 6(6H) (Fig.