| Acronym | Definition |
|---|
| SPOT | Système Pour l'Observation de la Terre (French remote sensing satellite) |
| SPOT | Satellite Personal Tracker |
| SPOT | Spotlight |
| SPOT | Shared Product Object Tree |
| SPOT | Smart Personal Object Technology (Microsoft) |
| SPOT | Single Point of Truth |
| SPOT | Screening of Passengers by Observation Techniques |
| SPOT | Satellite Probatoire d'Observation de la Terre |
| SPOT | Sensor Placement Optimization Tool (drinking water research) |
| SPOT | Synchronized Predeployment and Operational Tracker (US Department of Defense program for tracking deployed contractors) |
| SPOT | Special Purpose Operational Training (airlines) |
| SPOT | Single Payment Options Trading (securities) |
| SPOT | Synchronized Pre-Deployment and Operational Tracker (bar-code system used to track travel/movement of DoD contractors) |
| SPOT | Sequential Proxy Optimization Technique |
| SPOT | Secondary-Location Point of Termination |
| SPOT | Sociedad Puertorriqueña de Ortopedia y Traumatología |
| SPOT | Searching Peak of Tension (polygraph testing) |
| SPOT | Stupid Previous Owner Trick (automotive) |
| SPOT | Sensor Placement Orientation Tool |
| SPOT | Simply Protecting Our Tots |
| SPOT | Space Station Proximity Operations Trainer |
| SPOT | Speed Position and Track |
| SPOT | Sprint Procurement Online Tool |
| SPOT | Signaling Point of Termination |
| SPOT | Strategic Programs of Technology (Sprint) |
| SPOT | Swiss Professional Officer Training Course |