| Acronym | Definition |
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| SPOT | Système Pour l'Observation de la Terre (French remote sensing satellite) | | SPOT | Spotlight | | SPOT | Satellite Personal Tracker | | SPOT | Smart Personal Object Technology (Microsoft) | | SPOT | Shared Product Object Tree | | SPOT | Single Point of Truth | | SPOT | Screening of Passengers by Observation Techniques | | SPOT | Satellite Probatoire d'Observation de la Terre | | SPOT | Synchronized Predeployment and Operational Tracker (US Department of Defense program for tracking deployed contractors) | | SPOT | Special Purpose Operational Training (airlines) | | SPOT | Single Payment Options Trading (securities) | | SPOT | Synchronized Pre-Deployment and Operational Tracker (bar-code system used to track travel/movement of DoD contractors) | | SPOT | Sequential Proxy Optimization Technique | | SPOT | Secondary-Location Point of Termination | | SPOT | Sociedad Puertorriqueña de Ortopedia y Traumatología | | SPOT | Searching Peak of Tension (polygraph testing) | | SPOT | Stupid Previous Owner Trick (automotive) | | SPOT | Sensor Placement Orientation Tool | | SPOT | Simply Protecting Our Tots | | SPOT | Space Station Proximity Operations Trainer | | SPOT | Speed Position and Track | | SPOT | Sprint Procurement Online Tool | | SPOT | Signaling Point of Termination | | SPOT | Strategic Programs of Technology (Sprint) | | SPOT | Swiss Professional Officer Training Course |
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