Negotiating diverse viewpoints, a main element of TGCE, is not apparent in this video.
Second, while the Bridges' curriculum lacked a strong focus on the politics/ideology dimension of TGCE, the digital narratives themselves could be used to counter the stereotypes that students have of distant places and people portrayed in mass media (Niens & Reilly, 2012).
Examples of the TGCE output showing the detection of heteroduplexes in mixed specimens are presented in Figs.
3 in the online Data Supplement) was digested with the appropriate restriction enzyme (Table 1 in the online Data Supplement) to generate smaller fragments that could be individually resolved by TGCE (Fig.
There were no false positives identified in this TGCE protocol when the Optimase DNA polymerase was used for amplification of the PCR products.
Two techniques have emerged that combine high-sensitivity heteroduplex detection with automated liquid handling for the process of mutation discovery: DHPLC and TGCE.
In our study, we evaluated the use of TGCE to screen for the presence of mtDNA sequence variations in human NMSC samples.
TGCE in the 96- or 384-well format offers substantial advantages over these techniques in terms of sample capability, which is limited by the number of wells and manual gel preparation for TTGE and by the serial nature of running DNA on a DHPLC column.
1] Nonstandard abbreviations: mtDNA, mitochondrial DNA; DHPLC, denaturing HPLC; TTGE, temporal temperature gradient gel electrophoresis; TGCE, temperature gradient capillary electrophoresis; NMSC, nonmelanoma skin cancer; SCC, squamous cell carcinoma; and BCC, basal cell carcinoma.