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TRICINEN-Tris(Hydroxymethyl) methylglycine
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A total of 20 [micro]g of crude venom and 10 [micro]g of venom fractions were separated under reducing-conditions in a 10-20% Tricine acrylamide gel.
Samples were separated under reducingconditions in a 10-20% Tricine acrylamide gel at 120V for 90 min and electrotransfered to a nitrocellulose membrane.
The Rubisco assay buffer included Tricine (100 mM, pH 8.
After washing, the supernatant were discarded and resuspended with buffer (50 mM Tricine, 10 mM MgS[O.
The second dimension was run at 150 V for ~60 min with 100 mmol/L Tris, 100 mmol/L tricine, 10 g/L SDS as cathode buffer (pH 8.
Only one band could be detected in serum from a patient with confirmed bone metastases by Western blot when using a 20% tricine gel.
The substrate solution contained 20 mmol/L tricine, 1.
The "Tricine" buffer contained 50 mmol/L Tricine adjusted with sodium hydroxide to pH 8.
Melatonin and indole analogs, bovine serum albumin (BSA; A-8022), HSA (A-1653), human [gamma]-globulin (G-4386), incomplete Freund's adjuvant, Iodogen (1,3,4,6-tetrachloro-3[alpha],6([beta]-diphenylglycouril; T-0656), Tricine (T-0377), and normal rabbit serum (R-9133) were purchased from Sigma Chemical Co.
For exon 9, Excelgel and sodium dodecyl sulfate buffer 1200 mmol/L Tricine [N-tris(hydroxymethyl)methylglycine], 200 mmol/L Tris, 5.