FWGE affects a strong inhibition of transketolase
(TK), slowing glucose uptake into pancreatic cancer cells and inhibiting the cells' synthesis of RNA.
We hope in the future to include other diagnostic tests, for example, serum vitamin B1 levels, RBC transketolase
activity, homocysteine, methylmalonic acid, and blood vitamin B6 levels.
Thiamine and its dependent enzymes, transketolase
(TK), pyruvate dehydrogenase, a-ketoglutarate dehydrogenase and the branched chain a-ketoglutarate dehydrogenase complex are involved in the maintenance of NADPH levels and carbohydrate metabolism in the cell8.
The enhanced expression of transketolase
-like 1 (TLKL-1) protein--the only transketolase
that is overexpressed in cancer--was shown to correlate with a more malignant RCC phenotype in a study by Langbein and colleagues.
plays a role in a chain of reactions called the pentose phosphate pathway, whereas PDH and KGDH are involved in glycolysis and the citric acid cycle.
During periods of high metabolic demand and high glucose intake, thiamine is needed as a cofactor for several enzymes, including transketolase
, [alpha]-ketoglutarate dehydrogenase, and pyruvate dehydrogenase.
The measurement of erythrocyte transketolase
activity with co-enzyme stimulation was done on a Roche Cobas Fara centrifugal analyzer (Brownes, Reading, UK) by the method of Mount (8).
The nutritional status of thiamin is assessed by the erythrocyte transketolase
A diagnosis of thiamine deficiency can be supported by decreased levels of erythrocyte transketolase
, but it's important not to wait for lab results.
This finding, combined with low serum transketolase
levels in both patients, led these researchers to conclude that this complication developed due to thiamine deficiency.
In addition, the supplement group also had improved transketolase
and aspartate transaminase status.
This study in Nature Medicine (published February 17, 2003 online edition) opens up the exciting possibility that easily absorbed lipid soluble thiamine effectively increases the activity of transketolase
and thus may have additional therapeutic uses, including prevention and treatment of the side effects of diabetes.