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UIDA |
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MLST was performed on the basis of the nucleotide sequence of 580- to 672-bp amplicons of 7 conserved housekeeping genes: aspC, clpX, fadD, icdA, lysP, mdh, and uidA. Specific primers were used: uidA (Bej, McCarty, & Atlas, 1991), Stx (Lin, Kurazono, Yamazaki, & Takeda, 1993), eaeA (Beaudry, Zhu, Fairbrother, & Harel, 1996), LT (Osek, Gallien, Truszezynski, & Protz, 1999), EAST1 (Yamamoto & Nakazawa, 1997), ehxA (Schmidt, Scheeff, Huppertz, Frosch, & Karch, 1999), and AAF1 (Monteiro-Neto, Campos, Ferreira, Gomes, & Trabulsi, 1996). Many are based on the UIDA gene, or the [beta]-D-glucuromdase (GLUase) enzyme for which it encodes. |
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