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Fish were sampled twice monthly during the spawning season (within 3 days of the full and new moons) at the US1 and DS1 sites (eight collections from 7 May through 28 September 2001, nine collections from 4 April through 20 September 2002) and approximately once monthly during the nonspawning season. US1 may exit the old group on November 15, 2004, and not become a member of an existing new group. 2], 2 U of Ampli Taq Gold (Applied Biosystems) 25 pmol of the forward primer (HEVORF2con-s1; nt 6283-6306 in the HEV US1 strain). |
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