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Densitometry with a computerized image analyzer was used to quantify the results (GelDocIT System; UVP, Upland, CA, USA). Prior to founding 3D Systems, Hull served six years as vice president of engineering at UVP Inc. The gels were digitally imaged using a UVP Bio Doc-It camera (Ultra-Violet Laboratory Products, Upland, CA, USA), and the concentration of each PCR product was determined by comparing the intensity of each band to a standard curve derived from a low DNA mass ladder (Invitrogen Corp. |
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