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| Acronym | Definition |
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| USO | United Service Organizations (provides morale, welfare and recreation-type services to US uniformed military personnel) | | USO | United States Oil Fund (stock symbol) | | USO | Unión Sindical Obrera (Spanish: Workers Syndical Union; Spain) | | USO | Universal Service Obligation | | USO | Ultra Stable Oscillator | | USO | United States Open | | USO | Universal Service Order | | USO | Unilateral Salpingo-Oophorectomy (surgical procedure) | | USO | United Stars Organisation (science fiction) | | USO | Universitair Symfonisch Orkest (University Symphony Orchestra; Leuven, Belgium) | | USO | Unidentified Submarine Object | | USO | Unit Security Officer | | USO | Unidentified Submersible Object | | USO | Unique Sink Orientation | | USO | Unix Software Organization | | USO | Union Sportive Oyonnax (French sports organization) | | USO | Union Sportive Orléans (French sports organization) | | USO | Unidentified Superconducting Object | | USO | Utsjoen Sähköosuuskunta (electric supplier company of Utsjoki, Finland) | | USO | Unidentified Sliding Object (vehicle) | | USO | Undercover Special Operations | | USO | Utah Symphony Orchestra (est. 1940) | | USO | University Symphony Orchestra (various universities) | | USO | United Solar Ovonic (various locations) |
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