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VMRD

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VMRDVeterinary Medical Research and Development, Inc.


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Diagnoses were made by serologic testing with commercial test kits (Pourquier ELISA Bluetongue Serum, Institut Pourquier, Montpellier, France; Bluetongue Virus Antibody Test Kit, VMRD, Pullman, WA, USA; ID Screen Blue Tongue Competition ELISA Kit, ID Vet, Montpellier, France; INGEZIM BTV, INGENASA, Madrid, Spain) or real-time reverse transcription-PCR (RT-PCR) (6).
Total IgA, IgG, and IgM concentrations were determined using single radial immunodiffusion assay kits (243-30, 240-30, and 246-30; VMRD Inc.
Diagnostic testing was performed with commercially available BTV-blocking-ELISAs (Pourquier ELISA Bluetongue Serum, Institut Pourquier, Montpellier, France; Bluetongue Virus Antibody Test Kit, VMRD, Pullman, WA, USA: ID Screen Bluetongue Competition ELISA kit, ID Vet, Montpellier, France; INGEZIM BTV, INGENASA, Madrid, Spain) and validated BTV real-time PCRs (12; B.
 
 
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