Organizations such as the Western High Speed Rail Alliance and the WCX focus on regional infrastructure challenges.
The WCX offers an example that could be tailored to regional PPP units throughout the United States.
sup] In our previous study, WCX
was a superior means of finding differential proteins and establishing steady models.
To identify these proteins, we fractionated BALF from rats 24 hr postexposure by SPE using a WCX resin with subsequent denaturing polyacrylamide gel electrophoresis (SDS-PAGE).
The WCX extraction addresses both of these by reducing the concentration of the most abundant protein (albumin) and reducing the total number of proteins present.
We prepared samples as described previously (5, 26), with 100 [micro]L patient sample mixed with 100 [micro]L of 10 mmol/L ammonium phosphate buffer containing deuterated internal standards before being loaded onto an Oasis WCX [micro]Elution 96-well SPE plate.
3], MRM with multistage fragmentation; Q/LIT, quadrupole linear ion trap; WCX, weak cation exchange; SPE, solid-phase extraction; HILIC, hydrophilic interaction liquid chromatography; HMMA, 4-hydroxy-3-methoxymethamphetamine; EQA, external quality assurance; LLOQ, lower limit of quantification.
2) illustrate these observations by showing that, for patients with isoforms, the relative difference between the c-ELISA and WCX TOF-MS was higher when plotted for WCX-TOF-MS hepcidin-25 (Fig.
These 2 standards were found to have identical binding characteristics to the surface of the WCX beads.
chips in an 8-well bioprocessor format (Ciphergen) were chosen to allow a larger volume of serum for the chip array.
Surface-enhanced laser desorption/ionization (SELDI)-TOF-MS (11,12) was performed with WCX
chips (weak cation-exchange capacities), SAX chips (anion-exchange capacity), and immobilized metal-affinity capture (IMAC) chips (metal-chelating capacity).
Taylor added : 'The WCX
is working to solve one of the most pressing issues facing America today, which is to get critical infrastructure built and built right.