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YAC |
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In 1996 Resnick, with NIEHS colleagues Vladimir Larionov and Natalya Kouprina, perfected a method using yeast artificial chromosomes, or YACs, for the specific isolation of entire human genes. Using yeast artificial chromosome complementation and direct cDNA selection, two groups subsequently identified portions of a candidate gene for bg, named Lyst (lysosomal trafficking regulator) (77,78). Although investigators have made yeast artificial chromosomes for more than a decade (SN: 6/5/93, p. |
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