72) indicated a very strong relation between qPCR-measured ZAP70 expression and mutational status; the other markers showed a less good relation (0.
PROGNOSTIC VALUE OF QPCR-MEASURED ZAP70 EXPRESSION TFS AND OS ANALYSES
Need for treatment and patient death are clearly associated with both IGHV mutational status and qPCR-measured ZAP70 expression (Table 1).
We observed a significant difference in TFS only for qPCR-measured ZAP70 expression.
and FC-measured ZAP70 expression were the only significant predictors of OS (P = 0.
The AUC for ZAP70 expression (by either method) was higher than for any of the other prognostic factors, including mutational status (Table 2 and Fig.
Cross-tabulations for IGHV mutation status and ZAP70 and LPL expression are summarized in Table 1.
We also assessed the distinguishing power of ZAP70 and LPL expression with performance variables such as sensitivity, specificity and positive and negative predictive values (Table 2).
3A) revealed a significant association between LPL and ZAP70 expression [68%: [chi square](1) = 0.
A scatter plot of the percentage of cells positive for ZAP70 expression vs the ZAP70 qPCR result for 57 patients (Fig.
All 5 CLL-related deaths occurred in the patient subgroup characterized by unmutated IGHV genes and by ZAP70 and LPL expression positivity.
Gene expression studies have suggested a number of alternatives, with ZAP70 expression being among the most promising (7, 8).