The expression of proteins (claudin-5, ZO-1, and occludin) was analyzed by a two-step EnVision/horseradish peroxidase (HRP) technique, following the manufacturer's instructions.
Compared with Group A, the relative mRNA expressions of ZO-1 in Group B ( t = 11.
The results showed that compared with the control, the expressions of occludin, claudin-1 and ZO-1 mRNA decreased by 46%, 57%, and 34% respectively in the 100 [micro]g/mL LPS -treated group (p<0.
As depicted in Table 3, compared with the control, the addition of GLP-2 (100 nmol/L) increased the expressions of Akt, mTOR, occluding, claudin, and ZO-1 mRNA (p<0.
Altered expression and localization of the tight junction protein ZO-1 in primary and metastatic pancreatic cancer.
Different expression of occludin and ZO-1 in primary and metastatic liver tumors.
Figure 3 showed the expression of ZO-1 intestinal tight junction.
Using CISH technique showed that intestinal tight junction express mRNA ZO-1 after LPS induced rats getting probiotic LIS 20506 supplementation for 3 days (Figure4).
The decreased mRNA and protein expression levels of claudin-5, occludin, and ZO-1
in microvessel fragments of cerebral ischemic tissue were significantly prevented by treatment with GTPs at the same time points after ischemia in rats.
We studied two major proteins involved in the tight junctions of BBB, ZO-1
Compared with the control, the expressions of ERK1, ERK2, p90RSK, ZO-1
, claudin-1, occludin mRNA increased 85%, 195%, 47%, 29%, 120%, and 54% respectively in the 10 nmol/L GLP-2-treated group and 301%, 747%, 883%, 59%, 251%, and 142% respectively in the 100 nmol/L GLP-2-treated group while the abundance of total ERK1/2 protein was unchanged (p>0.
The expression of ZO-1
and occludin was upregulated in the cells treated with LTA-BS or B.