| Acronym | Definition |
|---|
| DFO | Department of Fisheries and Oceans (Canada) |
| DFO | Designated Federal Official |
| DFO | Disaster Field Office (US FEMA) |
| DFO | Derived Format Object |
| DFO | Dispatcher Failover |
| DFO | Data Flow Operator |
| DFO | Data Flow Object |
| DFO | Darkfall Online (online gaming) |
| DFO | Deferoxamine |
| DFO | Divisional Forest Officer |
| DFO | Dairy Farmers of Ontario (Canada) |
| DFO | Director of Financial Operations (various organizations) |
| DFO | Director of Flight Operations (airline rank) |
| DFO | Distillate Fuel Oil |
| DFO | Direct Factory Outlet (Australian retail center) |
| DFO | District Forest Office |
| DFO | Dartmouth Flood Observatory |
| DFO | Drilling Fluid Operator (offshore oil & gas) |
| DFO | 1,8-Diazafluoren-9-One (forensic chemical) |
| DFO | Director of Field Operations |
| DFO | Dolphins Fanatics Online (football website) |
| DFO | Done Fell Out |
| DFO | Dislodged Foreign Object (gaming; World of Warcraft) |
| DFO | Deutscher Falkenorden (German Falcon Order) |
| DFO | Des Filles en Ovalie (French: Girls in Rugby) |
| DFO | Diesel Fuel Oil |
| DFO | Digital Fuel Optimizer (RevTech motorcycle fuel control system) |
| DFO | Dell Factory Outlet |
| DFO | Donor Funded Operation (World Bank) |
| DFO | Date File Opened (US DHS) |
| DFO | Done Fell Over |
| DFO | Dining Facility Operation |
| DFO | Distance from Origin |
| DFO | Dungeon Fighter Online (video game) |
| DFO | Divisional Finance Officer |
| DFO | Documentation for Operations |
| DFO | Duty Field Officer (UK) |
| DFO | Dumb Freakin' Operator (polite form) |
| DFO | Diploma in Front Office (various educational institutions) |