| Acronym | Definition |
|---|
| DOT | Department Of Transportation |
| DOT | Dictionary Of Occupational Titles |
| DOT | Department of Transport |
| DOT | Division of Transportation (various locations) |
| DOT | Department Of Telecommunications |
| DOT | Directly Observed Therapy |
| DOT | Director of Technology |
| DOT | Document Template (filename extension for Microsoft Word template) |
| DOT | Damage Over Time (gaming) |
| DOT | Door of Time (gaming) |
| DOT | Department of Trade |
| DOT | Department of the Treasury |
| DOT | Direction of Travel |
| DOT | Director Of Training |
| DOT | Department of Tourism |
| DOT | Days of Treatment |
| DOT | Description of Test (careers) |
| DOT | Delivered on Time |
| DOT | Days of Thunder |
| DOT | Diffuse Optical Tomography |
| DOT | Deed of Trust |
| DOT | Distributed Object Technology |
| DOT | Discovery on Target (medical discovery conference) |
| DOT | Défense Opérationnelle du Territoire (French: Operational Territorial Defense) |
| DOT | Date of Termination |
| DOT | Designated Order Turnaround (NYSE trading method) |
| DOT | Directly Observed Treatment (tuberculosis) |
| DOT | Direct Operation Technology |
| DOT | Document Template |
| DOT | Dynamic Overclocking Technology |
| DOT | Duration Of Therapy |
| DOT | Digital Opportunity Task Force |
| DOT | Dutch Open Telescope |
| DOT | Disodium Octaborate Tetrahydrate |
| DOT | Dicapo Opera Theatre (New York, NY) |
| DOT | Dedicated Offshore Team |
| DOT | Distribution Oriented Technologies (Jordan, Amman) |
| DOT | Diocese of Torit |
| DOT | Date of Theft |
| DOT | Druid of the Talon (video game character) |
| DOT | Directory of Trades |
| DOT | Designating Optical Tracker |
| DOT | Design on Textile |
| DOT | Digital Optical Tape |
| DOT | Dose Optimized Thermotherapy |
| DOT | Deployment Operations Team |
| DOT | Dynamic Object Technology |
| DOT | Director On Target |
| DOT | Digital Oligonucleotide Tiling |
| DOT | Division Officer Training |
| DOT | Date of Transplanting |
| DOT | Deep Ocean Technology/Transponder |