The phenomenon of induced
electroosmotic flow mainly depends on the interaction of polarizable solids with an applied electric field to generate an electromotive phenomenon.
What is more, the retention time was prolonged, which might be attributed to the fact that the higher buffer concentration resulted in the reduction of
electroosmotic flow. Herein, the orthogonal test was carried out by three levels of [H.sub.3]B[O.sub.3]-[Na.sub.2][B.sub.4][O.sub.7] buffer (30, 35, and 40 mmol/L).
A sustained and variable
electroosmotic flow was observed due to the migration of the [Na.sup.+] ions, which could enhance the migration of the double layer complexes toward the cathode, while nitrate ions could be involved in complex formation with the cations [28].
As known, the buffer concentration acts directly on the magnitude of the
electroosmotic flow (EOF), and a higher buffer concentration gives a low EOF [17-19].
Electroosmotic flow results from the action of the tangential component of the electric field on the diffuse double layer forming at the electrode-medium interface.
Numerical simulation of
electroosmotic flow. Analytical Chemistry 70(9):1870-81.
Pikal, The role of
electroosmotic flow in transdermal iontophoresis, Adv.
Thus, the imidazole-containing zwitterionic salt covalently attached to the silica capillary wall was characterized by the cathodic
electroosmotic flow and by an almost constant velocity in the pH range of 4 to 7.
From the inlet reservoir the sample is driven into the channels for analysis by either electromigration or
electroosmotic flow. We developed a sample collector that enabled us to measure samples from patients as well as whole blood from volunteers on the same microchip.
The following Helmholz-Smoluchowski equation is often used to estimate the average
electroosmotic flow velocity ([V.sub.eo]):
Based on the neutral marker (labeled "EOF" for
electroosmotic flow), the [[mu].sub.ep]'s of the species were calculated to be -1.41, -1.56, and -1.92 x [10.sup.-4] [cm.sup.2]*[V.sup.-1]*[s.sup.-1], respectively, where the negative sign indicates a mobility vector opposing the
electroosmotic flow direction.