| Acronym | Definition |
|---|
| OWL | Online Writing Lab |
| OWL | Overwatch League (gaming clan) |
| OWL | Web Ontology Language (W3C) |
| OWL | Opening the World of Learning (curriculum) |
| OWL | Ostwestfalen-Lippe (German region) |
| OWL | Our Whole Lives (Unitarian Universalist youth groups) |
| OWL | Olympic Weightlifting (sport) |
| OWL | Older Women's League (Washington, DC) |
| OWL | Ongoing Weight Loss (Atkins diet) |
| OWL | Orthopaedic Web Links |
| OWL | Open Windows Library |
| OWL | Web Ontology Language |
| OWL | Oxford Wireless Lan |
| OWL | Outpost Firewall |
| OWL | Other Wooded Land (classification) |
| OWL | Ontology Web Language |
| OWL | Outdoor Webelos Leader (Boy Scouts) |
| OWL | Object Windows Library |
| OWL | Online Web Library |
| OWL | Overwhelmingly Large Telescope |
| OWL | Ordinary Wizarding Level (Harry Potter books) |
| OWL | Organization-Wide Learning |
| OWL | Obsessed With Learning |
| OWL | Optical Wallet Light (magnifier) |
| OWL | Outlined White Letters (Refers to outlined white-letters on the sidewalls of certain tires) |
| OWL | Orbiting Wide-Angle Light Collector |
| OWL | Omaha Worship Leaders (Omaha, NE) |
| OWL | Obfuscated Weird Language (character-based language interpreter) |
| OWL | Outdoors Without Limits |
| OWL | Open-Wire Line |
| OWL | Open Space, Water Resource Protection and Land Use |
| OWL | Operator Wireless and Line |
| OWL | Obstacle Warning Laser |
| OWL | Order of the White Lotus (animated TV show Avatar: The Last Airbender) |
| OWL | Older & Wiser Lutheran |
| OWL | Online with Laura (golf) |
| OWL | One Way Landers |
| OWL | Object Work List (programming) |
| OWL | Outstanding Working List (manufacturing) |