| Acronym | Definition |
|---|
| ROT | Republic of Texas (biker rally) |
| ROT | Rule of Thumb |
| ROT | Reign of Terror (band, and also a gaming clan) |
| ROT | Root of Trust (computing) |
| ROT | Rotunda (Portuguese postal usage) |
| ROT | Risk of Trip |
| ROT | Right on Time (Red Hot Chili Peppers song) |
| ROT | Right on Time |
| ROT | TAROM Romanian Air Transport (ICAO code) |
| ROT | Rotation |
| ROT | Restraint of Trade |
| ROT | Running Object Table |
| ROT | Rate of Turn |
| ROT | Registered Orthopedic Technologist (American Society of Orthopedic Professionals) |
| ROT | Return on Talent |
| ROT | Rule of Thirds (photography) |
| ROT | Republic of Turkey |
| ROT | Retention of Title |
| ROT | Rose of Tralee (Ireland) |
| ROT | Rise of the Triad (video game) |
| ROT | Really Off Topic |
| ROT | Redundant, Obsolete, Trivial |
| ROT | Runway Occupancy Time |
| ROT | Remaining Operating Time |
| ROT | Retailers' Occupation Tax (Illinois, USA) |
| ROT | Redundant, Outdated, or Trivial (material on web pages; US EPA) |
| ROT | Record Of Trial |
| ROT | Remotely Operated Tool |
| ROT | Receive Only Terminal |
| ROT | Rise Over Thermal (wireless communication systems) |
| ROT | Right Offensive Tackle (football position) |
| ROT | Rehabilitate-Own-Transfer (project/financing arrangement) |
| ROT | Rise of Tyrants (online game) |
| ROT | Registered Option Trader |
| ROT | Requirements Operability Test (US Air Force) |
| ROT | Rise of Terror (online game) |
| ROT | Rolled on Tube (textile business) |
| ROT | Revision of Terms (loans) |
| ROT | Return on Turnover |
| ROT | Realms of Thoth (gaming) |
| ROT | Radar On Target |
| ROT | Results Oriented Thinking |
| ROT | Reapers of Time (gaming clan) |
| ROT | Right on Test |
| ROT | Resource Occupancy Time |
| ROT | Residue Off-Site Treatment |
| ROT | Reign of Tatooine (gaming clan) |
| ROT | Radar-Only Tactic |
| ROT | Royal Operating Table (Crossing the Line Ceremony) |