| Acronym | Definition |
|---|
| TFO | Tritium/Fiber Optic (gun sight) |
| TFO | Temple Fork Outfitters (Dallas, TX) |
| TFO | The Florida Orchestra |
| TFO | Trouble Free Operation |
| TFO | Tandem Free Operation |
| TFO | Trust Fund Operations (World Bank) |
| TFO | Triplex Forming Oligonucleotides |
| TFO | Tactical Flight Officer |
| TFO | Task Force Officer |
| TFO | Tijdschrift Fiscaal Ondernemingsrecht (Dutch: Journal of Corporate Tax Law) |
| TFO | Transcoder Free Operation |
| TFO | The Funeral Orchestra (band) |
| TFO | Transfer of Functions Order (UK) |
| TFO | Terror-Free Oil (initiative) |
| TFO | Tesi Filosofiche Online |
| TFO | The Fabulous One |
| TFO | The Furniture Ombudsman (UK) |
| TFO | Transport Flow Optimization |
| TFO | Too Freaking Old |
| TFO | Task Force Orange |
| TFO | Township Fiscal Officer |
| TFO | Transactions For Others |
| TFO | Task Force Olympia (Operation Iraqi Freedom) |
| TFO | Télévision Francophone en Ontario (French: French-Language Television in Ontario; Ontario, Canada) |
| TFO | Tenant Fit Out |
| TFO | Task Force Organisation (France) |
| TFO | Tactical Fiber-Optics |
| TFO | Totally Freaked Out |
| TFO | Toll Fraud Operations (Sprint) |
| TFO | Texas Finish Out Construction (Grapevine, TX) |
| TFO | Trauma from Occlusion (dental) |