Primers: For PCR amplification of VP4 and
VP7 genes of RV, previously published primers were used (Gentsch et al.
Los rotavirus poseen dos proteinas de capside externa VP4 y
VP7, que estimulan la produccion de anticuerpos neutralizantes de manera independiente; en base a estas dos proteinas, dichos virus se han clasificado en serotipos P por la VP4 y serotipos G por la
VP7 [21].
(5) G and P genotyping is attributed, respectively, to
VP7 and VP4.
The
VP7 genes of the human G8P[8] strains isolated in this study and in Southeast Asia appear to have a close relationship with bovine strains from Asia but not from Japan, and the
VP7 gene of human G8 or bovine G8 strains previously isolated in Japan are distantly related to them.
Given that reassortment is a major feature of orbivirus evolution (30,31), we further explored the evolutionary relationships between the 55 AHSV sequences by constructing separate maximum-likelihood trees for each of the VP1, VP2, VP3, VP4, VP5, VP6,
VP7, nonstructural (NS) protein 1 (NS1), NS2, and NS3 encoding genome segments (online Technical Appendix 2 Figures 1-10).
Isolates from the 4 samples were identified as genotype G9P[8] and found to have identical VP4 and
VP7 sequences (GenBank Accession nos.
The genome of RVA consists of 11 double-stranded RNA segments that code for 11 or 12 viral proteins (VP1-VP4, VP6,
VP7, nonstructural protein 1 [NSP1]-NSP5/6) (2).
The outer capsid proteins,
VP7 and VP4, elicit neutralizing antibodies.