Note: C/EBP[alpha], CCAAT/Enhancer Binding Protein Alpha; DBT, dibutyltin; DMSO,
dimethylsulfoxide; Fabp4, fatty acid binding protein-4; Fsp27, fat-specific protein-27; LPL: lipoprotein lipase; h/mMSCs, human/mouse mesenchymal stem cells; PPAR[gamma]2, peroxisome proliferator-activated receptor gamma; ROSI, rosiglitazone; SEM, standard error of the mean; TBT, tributyltin.
Interestingly, the assembly of vimentin microfibrils in MADB fibroblasts improved with rapamycin and
dimethylsulfoxide.
So, flurbiprofen was dissolved in undiluted
dimethylsulfoxide (DMSO).
For example, a co-extruded product line can safely deliver
dimethylsulfoxide in such a way that it does not absorb into the tube itself and holds up under long-term exposure, with no detectable tube degradation.
The concentration of the cryoprotectants
dimethylsulfoxide (DMSO) was 10%, dilution ratio 1:3 (v/v) and cooling rate 60[[degrees]C.sup.-min].
The test was terminated by the addition of 0.6 mM phloretin (250 [micro]L, in Earle's/HEPES buffer and 0.05%
dimethylsulfoxide) at 4[degrees]C.
Methylprotodioscin and dioscin were dissolved in
dimethylsulfoxide and treated in culture medium (final concentrations of
dimethylsulfoxide were 0.5%).
Then, the 200 [micro]L of supernatant culture medium were carefully aspirated and 200 [micro]L aliquots of
dimethylsulfoxide (DMSO) were added to each well to dissolve the formazan crystals after incubation for 10 min to dissolve air bubbles, the culture plate was placed on a Biotek micro-plate reader and the absorbance was measured at 550 nm.