| Acronym | Definition |
|---|
| OTF | Only the Family (band) |
| OTF | Orange Theory Fitness (health club) |
| OTF | On-The-Fly (data acquisition method) |
| OTF | On-the-Fly |
| OTF | On the Floor (laughing) |
| OTF | Out the Front (automatic knife) |
| OTF | Ontario Trillium Foundation (Canada) |
| OTF | Off Topic Forum |
| OTF | on the Fly |
| OTF | Open Type Font |
| OTF | Open Tape Format |
| OTF | Organised Trading Facility (EU) |
| OTF | Open Type Fonts (Adobe) |
| OTF | Optical Transfer Function |
| OTF | Output Text Format (computer programming) |
| OTF | On the Phone (chat) |
| OTF | Ontario Teachers' Federation (Canada) |
| OTF | Off The Film (camera technology) |
| OTF | Over the Fence |
| OTF | Ohio Turfgrass Foundation |
| OTF | Open Token Foundation |
| OTF | One Time Fee |
| OTF | Off the Front (cycling/racing slang) |
| OTF | Open Trace Format |
| OTF | Over the Falls (surfing) |
| OTF | Optical Tunable Filter |
| OTF | Ohio, Texas, Florida (radio show) |
| OTF | Open Telecommunications Framework (ECTF) |
| OTF | Only the Facts |
| OTF | Object Testing Framework |
| OTF | Oral Thin Film (drug delivery) |
| OTF | Objective Terrain Format (high resolution database format) |
| OTF | Organ Transplant Fund |
| OTF | One Time Flight (US Air Force) |
| OTF | OneTouchFootball (soccer discussion site) |
| OTF | Outsider Test for Faith (religion) |
| OTF | Operate to Failure (engineering maintenance strategy) |
| OTF | Optimum Traffic Frequency |
| OTF | Offender Tracking Form |
| OTF | Out There Flapping |
| OTF | One Touch Financial |
| OTF | Orders To Fire |
| OTF | Optimized Thomas-Fermi |
| OTF | Optimal Tower Field |
| OTF | One True Friend |
| OTF | Orders To Fly |
| OTF | Optimal Tight Frame |
| OTF | Officially TB (Tuberculosis) Free (cattle herds; UK) |
| OTF | Order of the Federation (gaming clan) |
| OTF | On-Line Tax Form |