Restriction fragment length polymorphism, Single-nucleotide polymorphism
DNA Amplification and
Restriction Fragment Length Polymorphism (RFLP) Analysis
Chi et al., "Differentiation of Helicobacter pylori strains directly from gastric biopsy specimens by PCR-based
restriction fragment length polymorphism analysis without culture," Journal of Clinical Microbiology, vol.
were detected in the specimens and differentiated by PCR and
restriction fragment length polymorphism analysis of the small subunit rRNA gene (11).
Variation in
restriction fragment length polymorphism could be seen.
It has several known allelic variants including a FokI
restriction fragment length polymorphism in exon II, BsmI and ApaI polymorphisms in the intron between exons VIII and IX, and a mononucleotide [[(A).sub.n]] repeat polymorphism in the 3' untranslated region (Zmuda et al 2000).
Amplification and
restriction fragment length polymorphism (RFLP): PCR amplification and RFLP protocols for msp-3[alpha] were reported elsewhere (13).
The rapid methods used for identification include high performance liquid chromatography (HPLC) (5), DNA probes (Accuprobe; Gen-Probe, Inc, San Diego, Calif.),
restriction fragment length polymorphism (RFLP) using various target regions including hsp 65 (6), ITS (7), rpo B (8) and dna J gene (9).