In order to bring laboratory personnel up to speed with mass spectrometry, the American Association for Clinical Chemistry has established a new nine-course certificate program in using
tandem mass spectrometry in the clinical laboratory.
[2] Nonstandard abbreviations: LC-MS/MS, liquid chromatography coupled to
tandem mass spectrometry; RMP, reference method procedure; cRF, contemporaneous response function; sRF, sporadic RF.
Dass starts with the basics of operation and then covers modes of ionization, mass analysis and ion detection,
tandem mass spectrometry, hyphenated separation techniques, organic mass spectrometry, inorganic mass spectrometry, structure determination of proteins and peptides and their post-translational modifications, higher order structures, oligosaccharides, characterizations of lipids, and structure determination of oligonucleotides.
Tandem mass spectrometry provided confirmation of PbTx-3, which also increased for the time points between 0.5 and 4.0 hr exposure.
Successful and cost-efficient replacement of immunoassays by
tandem mass spectrometry for the quantification of immunosuppressants in the clinical laboratory.
Topics include using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition, stable isotope labeling by amino acids in cell culture for quantitative proteomics, quantification of proteins and metabolites without isotopic labeling, an isotope coding strategy for proteomics involving both amine and carboxyl group labeling,
tandem mass spectrometry in the detection of inborn errors of metabolism for newborn screening, computational analysis of quantitative proteomics data using stable isotope labeling, and quantitative proteomic analysis of phosphotyrosine-mediated cellular signaling networks.