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The slopes of increasing fluorescence based on real time PCR with the iCycler were indistinguishable from the increasing peak areas on the electropherogram (Figure 4, panels A and B, slopes of 0. The first stage of the agreement will see technical development to automate the export of electropherogram data from Agilent's bioanalyzer Expert software for analysis with Nonlinear's TotalLab TL120 DM for the purpose of varietal identification. Electropherograms were analyzed with Genemapper U 3. |
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