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Cytopathic effects (CPEs) or positive hemadsorption tests were confirmed by using immunofluorescence assays performed with monoclonal antibodies against HPIV-4 (Chemicon International, Temecula, CA, USA). If no effect was observed, the HeLa tubes were passaged blind at weekly intervals, and monkey kidney tissue cultures were tested for hemadsorption with guinea pig erythrocytes. cell culture tests for the presence of certain cytocidal viruses (by inoculation of and blind passage in Vero, MRC-5, HeLa, RK, and A9 cells with observations for cytopathology and tests for hemadsorption and hemagglutination at the end of the culture period), and animal inoculation tests for certain viruses (suckling and adult mice, guinea pigs, and embryonated hen eggs). |
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