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That codon makes the amino acid isoleucine, just as its counterpart in the most common form of the pump gene does. These strains had amino acid changes at position 351 in the E gene (from isoleucine to valine) and position 52 in the NS1 gene (from lysine to arginine), which supports their inclusion in the modern Caribbean basin clade (8). I have also supplemented with the essential amino acids--methionine, phenylalanine, tryptophan, leucine, isoleucine, valine, lysine, and threonine--but only after the previously listed supplements have been given. |
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