mFA1Murine Fetal Antigen 1
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CE-SSCP test under nondenaturing conditions with 3% GeneScan polymer at 30[degrees]C allowed characteristic peak patterns to be detected in MF[alpha]1 and MFa1 genes because of the individual differences within the nueleotide sequences.
Based on our success with MF[alpha]1 sense primer in the detection of characteristic peaks for CnVG, CnVN, and Cg, we labeled Mfa1 sense strands and analyzed MATa strains.
Thus, CE-FLA alone leads to visualization of size differences in MF[alpha]1 versus Mfa1 pheromones, which would distinguish mating types and hybrids.