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MOSM |
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The commercial Percoll (Sigma) solution was adjusted to 1,100 mOsm by adding NaCl to a final concentration of 0. Temperature in the organ bath was maintained at 24[degrees]C, and the osmolality of the bath was approximately 290 mOsm. marinus in 910 mOsm synthetic seawater (isotonic seawater) or 100-[micro]L isotonic seawater via mantle cavity injections with a 1-ml syringe and a 1", 25-gauge needle. |
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