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It has been shown that inoculation of murine cytome-galovirus-expressing mouse ZP3 (mZP3) (2) or purified mZP3 protein expressed by vaccinia virus (12) induces an anti-ZP3 immune response and results in a strong immunocontraceptive effect in immunized mice.
Our previous studies showed that nasal immunization induced both IgA and IgG Ab responses to mZP3 in mouse models (27,28).