The compound isolated from the n-BuOH
fraction of Androsace umbellata MeOH extract was identified as Saxi A (Fig.
In Figure 2, the DPPH radical scavenging activities of various investigated extract n-Butanol and Ethyl acetate of Catha Edulis with age 50 years were in order of n-BuOH
Table 1: Antimicrobial activity of n-BuOH
extract at different concentrations on five bacteria and one fungus.
This assay as shown in Figure 5, indicated that both AcOEt and n-BuOH
extracts of P.
A previous study suggested phenolic compounds found in the n-BuOH
fraction as responsible for the vasodilating activity of C.
9 M); group III, rats receiving glucose (4 gikg) plus Et0Ac fraction (100 and 200 mg/kg); and group IV, rats receiving glucose (4 g/kg) plus n-BuOH
fraction (100 and 200 mg/kg); group V, rats receiving glucose (4 gikg) plus aqueous extract of green mate infusion (50, 100 and 200 mg/ml); group VI, rats receiving glucose (4 g/kg) plus aqueous extract of roasted mate infusion (50, 100 and 200 mg/ml) of
fraction (205g) was subjected to column chromatography on a silica gel column using mixtures of n-hexane-EtOAc-MeOH of increasing polarity as eluents to give 13 fractions (BI-BXIII).
2]O and partitioned with EtOAc and n-BuOH
successively, to afford EtOAc (120 g), n-BuOH
2], EtOAc and n-BuOH
successively, yielded four fractions, a petroleum ether fraction (yield 15.
japonica, the n-BuOH
and ethyl acetate fractions were subjected to repeated normal and reversed-phase Si gel column chromatography directed by in vivo bioassay.
The chloroform-insoluble residue obtained was then suspended in distilled water (500 ml) and then extracted in a separatory funnel with n-BuOH
saturated with water (300 ml x 8).
3], EtOAc and n-BuOH
were purchased from Sinopharm Chemical Reagent Co.