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HClO4 |
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After the 14-minute washes in the fixative, the cells were air dried, ribonucleic acid was removed, and the cells were treated for 24 hours in 5% perchloric acid at 4[degrees]C. All samples were assayed initially for total copper and cobalt by high temperature perchloric acid oxidative attack digestion with a hydrochloric acid final leach and AAS finish. 2]O:5% perchloric acid (5:1:1) and stirred at 22[degrees]C for 1 hr. |
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