rE7Recombinant E7 Protein
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ELISA and Western blotting with human sera: Each well of 96-well microtiter (NUNC, Germany) was coated overnight at 4[degrees]C with 100 [micro]l of the antigens; rE7 (5 [micro]g/ml), rNT-gp96 (2.
The rE7 migrated as a 23 kDa protein in SDS-PAGE (Fig.
There were significant differences between patient and controls in respect to rE7 and rCT-gp96 (P<0.
The bacterially expressed recombinant proteins of rE7 and rCT-gp96 appeared as a predominant band at 23 and 34 kDa respectively, but an extra band was also seen in the purified protein fractions (Fig.
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