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RNase |
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5 [micro]g oligo(dT), 40 U recombinant ribonuclease inhibitor RNaseOUT (Invitrogen, Carlsbad, CA, USA), and variable amounts of cellular RNA and recRNA. To determine the exact phylogenetic relationship of the new isolate within the genus Bartonella, we analyzed an alignment that contained the sequences of 3 important phylogenetic markers, ribonuclease P RNA (RNase P RNA), 16S rDNA, and 23S rDNA, merged by catenation and organized by secondary structure elements, as described (5). When he and his colleagues compared the two ribonuclease genes, they found several differences that change the enzyme's amino acids. |
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