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0), 1% nonionic detergent IGEPAL CA-630 (Sigma Chemical Company), 20 mM ethylenediaminetetraacetic acid, 150 mmol/L sodium chloride, 1 mmol/L magnesium chloride, 10 mM tetrasodium pyrophosphate, 100 mM sodium fluoride, 2 mM sodium orthovanadate, 10 [micro]g/mL leupeptin, 10 [micro]g/mL aprotinin, and 1 mM phenylmethylsulfonyl fluoride. Varied procedures in methylene blue and tetrasodium pyrophosphate preparation procedures. |
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