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Urine was collected directly from the bat's urogenital opening, before the blood sampling, with a cotton swab that was immediately placed in a cryotube containing 1 mL viral transport medium. The following chapter covers the trunk and the neck and is comprehensive and thorough for the movements in these areas, but somewhat superficial in its descriptions of the cardiovascular, respiratory, digestive, urogenital, and endocrine systems. Mice were examined daily for premature vaginal opening for the first 35 days of life and checked monthly with blunt forceps to detect concretions (calcium carbonate deposits in the vagina that are a result of malformation of the urogenital tract in developmentally estrogenized animals). |
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