Alpha-minimum essential medium ([alpha]-MEM), fetal bovine serum (FBS), dimethyl sulfoxide ([Me.sub.2]SO), antibioticantimycotic solution, phalloidin Alexa Fluor 488, 2,,7,-dichlorofluorescin diacetate (DCFH-DA), and 1,4-diazabicyclo
[2.2.2] octane (DABCO) were purchased from Invitrogen, USA.
1-Chloro-2,4-dinitrobenzene (CDNB), D-galactosamine (GalN), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 1,4-diazabicyclo
[2,2,2]octane (DABCO) were from Wako Pure Chemicals (Osaka, Japan).
After the rinse with PBSC under the same conditions again, larvae were mounted on a glass slide, and a drop of mounting medium (10% 1,4-diazabicyclo
[2.2.2] octane and 50% glycerin in PBSC) was added before a coverslip was placed over it.