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This technique replaced other bioassays for pregnancy, including follicle-stimulating hormone, and indirect hormone tests such as 17-ketosteroids.
The 2 most commonly used assays measure urinary excretion of PT, a urinary metabolite of 17OHP, or 17-ketosteroids (17-KS), a urinary metabolite of androgens.
Test tube extraction method for the microdetermination of urinary 17-ketosteroids.