The ethyl acetate and n-butanol fractions displayed clearly a stronger quenching activity (over 80%) against 2,2-diphenyl-1-picrylhydrazyl
(DPPH) and nitric oxide (NO) radicals, whereas all of them were observed with a lower activity (below 22%) against N,N-dimethyl-p-phenylendiamine (DMPD) radical.
This method uses the stable free 2,2-diphenyl-1-picrylhydrazyl
(DPPH) to estimate the activity of antioxidant.
pumila was first analyzed for their antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl
Galic acid, 2,2-diphenyl-1-picrylhydrazyl
(DPPH) and hydrochloric acid 32% were obtained from Sigma-Aldrich.
assay gave the highest antioxidant activity for EOOK compared to the oil from O.
0% pure, respectively, 2,2-diphenyl-1-picrylhydrazyl
, 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, potassium persulfate, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt, gallic acid and Folin and Ciocalteu's phenol reagent (Sigma--Aldrich, St.
0 mL of 2,2-diphenyl-1-picrylhydrazyl
(DPPH) solution in methanol(1.
They showed considerable free-radical-scavenging properties in the 2,2-diphenyl-1-picrylhydrazyl
(DPPH) assay with the RC (50) values of 9.
All reagents include Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl
, gallic acid, sodium carbonate, vitamin E, butylated hydroxyanisole, EDTA and all solvents (HPLC grade) were obtained from Fluka (Switzerland).
La capacidad antioxidante de las distintas muestras se evaluo como la capacidad de atrapar radicales utilizando la tecnica del radical libre 2,2-Diphenyl-1-picrylhydrazyl
(DPPH), siguiendo la metodologia propuesta por Brand-Williams et al.
The antioxidative capability of the compounds under study has been evaluated by using different assays like a ferric reducing/antioxidant power assay (FRAP), an oxygen radical adsorption capacity method (ORAC), colourization assays using stable coloured free radicals, such as 2,2-diphenyl-1-picrylhydrazyl
(DPPH), a 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation or a reactive oxygen species, due to their intensive absorbance in the visible region [2, 35-38].
Known methods include the active oxygen method (AOM) and its automated versions (Rancimat and SAFFEST) for lipophilic phases, and 2,2-Diphenyl-1-picrylhydrazyl
(DPPH) or oxygen radical absorption capacity (ORAC) for aqueous environments.