Antioxidants Analysis: Antioxidants before drying and retained in the samples after drying were determined using 2,2-diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging activity assay of mulberries.
radical scavenging assay
3 gas chromatography (Kyoto, Japan))by modified by Hara and Radin (1978), Christie (1992), 2,2-diphenyl-1-picrylhydrazyl
(DPPH) (The absorbance of the mixture was measured spectrophotometrically at 517 nm), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) (The absorbance of the mixture was measured spectrophotometrically at 734 nm), and malondialdehyde (MDA) (The equipment consisted of a pump (LC-10 ADVP), a UV-visible detector (SPD-10AVP), a column oven (CTO-10ASVP), an autosampler (SIL-10ADVP) a degasser unit (DGU-14A) and a computer system with class VP software (Shimadzu, Kyoto Japan) according to the method of Shimoi et al.
To each bottle 1.85ml of 0.05 mM ethanolic solution of DPPH (2,2-diphenyl-1-picrylhydrazyl
) was added.
All 2,4-dinitro phenyl hydrazone derivatives 1-32 were subjected to in vitro antioxidant activities, including DPPH (2,2-diphenyl-1-picrylhydrazyl
), hydroxyl radicals scavenging, ferrous ion-chelating, ferric reducing, total antioxidant activities.
The antioxidant activity was determined using the method of reducing the free radical DPPH (2,2-diphenyl-1-picrylhydrazyl
) based on the method proposed by Scherer and Godoy (2009), Rufino et al.
Other types of assays associated with the electron or proton donation mechanism include the 2,2-diphenyl-1-picrylhydrazyl
(DPPH) and ferric-reducing antioxidant power (FRAP) assay.
Due to their simplicity, the spectrophotometric methods based on reaction of the antioxidants with chromogenic radicals such as 2,2'-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid ([ABTS.sup.+]) and 2,2-diphenyl-1-picrylhydrazyl
(DPPH) or with phospho-tungsto-molybdate in Folin-Ciocalteu (FC) reagent are widely applied.
The aim of the research was in vitro evaluation of antioxidant potentials (neutralization of 2,2-diphenyl-1-picrylhydrazyl
(DPPH), hydroxyl (OH) and nitroso (NO) radicals, determination of reducing ability of ferric reducing potential (FRAP), and inhibition of lipid peroxidation) followed by preliminary chemical characterization of the horehound extract through quantification of the content of total phenolics and flavonoids.
The DPPH (2,2-diphenyl-1-picrylhydrazyl
) free radical scavenging method was performed and compared with standards like Vitamin E, BHT (Butylated hydroxytoluene) and ascorbic acid.
Another biological test done with warty Mimosa extract was radical sequestering qualitative test stable 2,2-diphenyl-1-picrylhydrazyl
In Vitro 2,2-Diphenyl-1-picrylhydrazyl
(DPPH) Free Radical Scavenger Activity.