In addition to 2-aminopurine, examined in the preceding section, those few loop base modifications are discussed here that have been carried out using the synthetic nucleotide analogs only, which increase or decrease the stability depending on the position of substitution.
Chaires, "Characterization of a [K.sup.+]-induced conformational switch in a human telomeric DNA oligonucleotide using 2-aminopurine fluorescence," Biochemistry, vol.
The s base is an analog of the well-known fluorescent 2-aminopurine
base, and the ribonucleoside of s exhibits fluorescence emission centered at 434 nm by excitation at 352 nm in phosphate buffer (pH 7).
Mutant libraries were generated using ethylmethanesulphonate (EMS), 2-aminopurine, and ultraviolet light.
Mutant libraries were generated using ethylmethanesulphonate (EMS), 2-aminopurine, and ultraviolet light in a mutagenic screen to study the affects of these mutations on the expression, secretion, and unipolar localization of IcsA.
The addition of 5-methylcytosine and 2-aminopurine
adjacent to the deletion improved discrimination dramatically.