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References in periodicals archive ?
The selectable marker 2-deoxy-D-glucose was found effective for such selection in earlier reports (Haq et al., 2005; Zia, 2007; Adsul et al., 2007) and hence, was used in this study.
[35] also used UV irradiation to isolate series of 2-deoxy-D-glucose resistant mutants from wild type Beauveria bassiana 88 (Bb 88).
Rajoka and Khan [37] isolated 2-deoxy-D-glucose and cycloheximide resistant mutant of Kluyveromyces marxianus PPY125 to study the production of [beta]-xylosidase in growth medium containing different carbon sources.
Barbati et al., "Differential effects of the glycolysis inhibitor 2-deoxy-D-glucose on the activity of pro-apoptotic agents in metastatic melanoma cells, and induction of a cytoprotective autophagic response," International Journal of Cancer, vol.
Shang et al., "Under normoxia, 2-deoxy-D-glucose elicits cell death in select tumor types not by inhibition of glycolysis but by interfering with N-linked glycosylation," Molecular Cancer Therapeutics, vol.
2-Deoxy-D-glucose was used at 1 mg mL-l for selection of mutant derived strains having the potential of enhance production of glucose oxidase.
It is reported that 2-deoxy-D-glucose is one of the best agent to screen and isolate the mutants with increased glucose oxidase activity (Gromada and Fiedurek, 1997), glucoamylase (Fiedurek et al., 1987) and cellulase (Labudova and Farkas, 1983).
Positron emission tomography (PET), which allows non invasive and quantitative analysis of various biologic processes, uses a glucose analogue (2-deoxy-D-glucose) labelled with a positron emitter Fluorine 18; FDG that is partially metabolized and trapped as its phosphate (2-DG-6-P) in the tumour tissue thus localizing the tumour (119).
In vitro and in vivo studies with several murine and human tumour cells have indeed shown that glycolytic inhibitors like 2-deoxy-D-glucose, 3-bromo-pyruvate etc., are selectively cytotoxic to tumour cells, inducing both growth inhibition and cell death (132).
The glucose analogue 2-deoxy-D-glucose (2-DG) is metabolized by hexokinase and acts as an inhibitor of glycolysis [25].
Uptake was initiated by addition of KRH supplemented with (1.0 ci) 2-deoxy-d-glucose [1,2-[.sup.3]H].