Effects of Rap and 3-MA
on ALI Secondary to Myocardial IR in Diabetic Rats.
The results were confirmed by 3-Ma
treatment (an autophagy inhibitor).
) inhibits the induction of autophagy by blocking the formation of autophagosomes and inhibiting class III phosphatidylinositol 3-kinases [20, 40].
In this study, the number of TUNEL-positive cells decreased in the presence of the autophagy inhibitor 3-MA
, whereas the number of TUNEL-positive cells was increased by the autophagy inducer rapamycin.
The MOVAS cells were randomly divided into the following groups: (1) control group, cells were incubated with DMEM; (2) ox-LDL group, cells were pretreated with ox-LDL (80 [micro]g/ml) for 72 h; (3) ox-LDL + low concentration rapamycin group, ox-LDL-pretreated cells were incubated with the mTOR inhibitor rapamycin (10 ng/ml) for another 24 h; (4) ox-LDL + high-dose rapamycin group, ox-LDL-pretreated cells were incubated with the mTOR inhibitor rapamycin (30 ng/ml) for another 24 h; and (5) ox-LDL + 3-MA
group, ox-LDL-pretreated cells were incubated with 3-MA
for another 24 hours, respectively.
To test whether the inhibition of autophagy can attenuate EMT in AA-treated HKC cells, 3-MA
, an inhibitor of autophagy, was used for the experiment.
We also pretreated A549/DDP cells with 3-MA
, a well-known inhibitor of autophagosomal lysosome formation, and found an abolished cell growth inhibition induced by cotreatment with high-dosage BZYQD and cisplatin (Figure 4(d)).
Inhibition of autophagy by 3-MA
enhances the effect of 5-FU-induced apoptosis in colon cancer cells.
The relative colocalization of PrP::GFP with Lyso-Tracker was, therefore, assessed following 16 h incubation with 2mM 3-methyladenine (3-MA
), a commonly used inhibitor of autophagy , or 2 [micro]M rapamycin, to induce autophagy .
Jurkat T cells were treated with rapamycin (an autophagy inducer), 3-methyladenine (3-MA
, an autophagy inhibitor), or DMSO (a vehicle control).
The 3methyladenine (3-MA
) (M9281; Sigma Co.) was dissolved in deionized water at 10 mM.
BSA and the inhibitor of autophagy 3-methyladenine (3-MA
) were purchased from Sigma-Aldrich (St.