(DAPI) staining was used to determine the nuclei.
After they were stained with 4',6-diamidino-2-phenylindole
(DAPI, 0.5 [micro]g/mL; Sigma, St Louis, Missouri) for 15 minutes and mounted, sections were observed using confocal laser scanning microscopy (CLSM) within 24 hours.
Dulbecco's phosphate-buffered saline with (DPBS) and without (DPB[S.sup.++]) calcium and magnesium, 50:50 Dulbecco's modified Eagle's medium with Ham's F-12 (DMEM/F12), Hank's balanced salt solution, penicillin-streptomycin-amphotericin B, insulin-transferrin-selenium A solution (ITS-A), TRIzol[R] reagent, High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, TaqMan gene expression assays, fetal bovine serum (FBS), trypsin ethylenediamine tetraacetic acid (EDTA), collagenase type IV, ProLong Gold[R] Antifade reagent with/without 4',6-diamidino-2-phenylindole
(DAPI), and rabbit anti-human CYP24A1 antibody were obtained from Thermo-Fisher (Waltham, MA).
(DAPI) nucleic acid stain (diluted at 1:5000 in distilled water; Thermo Fisher Scientific) was applied to the sections and washed.
Larvae were subsequently stained with antibodies, mounted onto slides in VECTASHIELD (Vector Laboratories, Burlingame, CA) with 4',6-diamidino-2-phenylindole
(DAPI), and photographed on a Nikon Eclipse E800 confocal fluorescent microscope or a Zeiss LSM 800 confocal microscope (Carl Zeiss, Jena, Germany).
The 2C=0.704-0.746 pg was found using DAPI (4',6-diamidino-2-phenylindole
) and PI (YAMANOUCHI et al., 2010).
Cytoskeleton and nuclei cells were stained with 50 [micro]g/mL rhodamine-phalloidin (Molecular Probes, USA) for 40 min and 0.5 [micro]g/mL 4',6-diamidino-2-phenylindole
(DAPI) for 5 min, respectively, for further imaging using a confocal microscope (Olympus FV1000, Japan).
Food and Drug Administration; EpCAM, epithelial cell adhesion molecule; PCa, prostate cancer; EMT, epithelial-to-mesenchymal transition; PSA-EPISPOT, prostate-specific antigen-enzyme-linked immunospotassay; IV, intravenous; RBC, red blood cell; FACS, fluorescence-activated cell sorting; laPCa, locally advanced prostate cancer; DAPI, 4',6-diamidino-2-phenylindole
The cells were incubated with VCAM-1 antibody (green) and nuclei were stained with 4',6-diamidino-2-phenylindole
The nucleus was stained with 4',6-diamidino-2-phenylindole
Nuclei were visualized using 4',6-diamidino-2-phenylindole
(DAPI) dihydrochloride (Sigma-Aldrich).
Slides were rinsed in PBS and counterstained with 4',6-diamidino-2-phenylindole