DAPI

(redirected from 4',6-diamidino-2-phenylindole)
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AcronymDefinition
DAPI4',6-Diamidino-2-Phenylindole (double stranded DNA staining)
DAPIDelaware Adolescent Program, Inc. (Wilmington, DE)
DAPIDimensional Assessment of Personality Impairment (psychiatric screening)
DAPIDestination Access Point Identifier
DAPIDefinity Application Programming Interface
DAPIDays After Panicle Initiation
DAPIDeveloper Application Programming Interface
References in periodicals archive ?
4',6-diamidino-2-phenylindole (DAPI) staining was used to determine the nuclei.
After they were stained with 4',6-diamidino-2-phenylindole (DAPI, 0.5 [micro]g/mL; Sigma, St Louis, Missouri) for 15 minutes and mounted, sections were observed using confocal laser scanning microscopy (CLSM) within 24 hours.
Dulbecco's phosphate-buffered saline with (DPBS) and without (DPB[S.sup.++]) calcium and magnesium, 50:50 Dulbecco's modified Eagle's medium with Ham's F-12 (DMEM/F12), Hank's balanced salt solution, penicillin-streptomycin-amphotericin B, insulin-transferrin-selenium A solution (ITS-A), TRIzol[R] reagent, High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, TaqMan gene expression assays, fetal bovine serum (FBS), trypsin ethylenediamine tetraacetic acid (EDTA), collagenase type IV, ProLong Gold[R] Antifade reagent with/without 4',6-diamidino-2-phenylindole (DAPI), and rabbit anti-human CYP24A1 antibody were obtained from Thermo-Fisher (Waltham, MA).
4',6-Diamidino-2-phenylindole (DAPI) nucleic acid stain (diluted at 1:5000 in distilled water; Thermo Fisher Scientific) was applied to the sections and washed.
Larvae were subsequently stained with antibodies, mounted onto slides in VECTASHIELD (Vector Laboratories, Burlingame, CA) with 4',6-diamidino-2-phenylindole (DAPI), and photographed on a Nikon Eclipse E800 confocal fluorescent microscope or a Zeiss LSM 800 confocal microscope (Carl Zeiss, Jena, Germany).
The 2C=0.704-0.746 pg was found using DAPI (4',6-diamidino-2-phenylindole) and PI (YAMANOUCHI et al., 2010).
Cytoskeleton and nuclei cells were stained with 50 [micro]g/mL rhodamine-phalloidin (Molecular Probes, USA) for 40 min and 0.5 [micro]g/mL 4',6-diamidino-2-phenylindole (DAPI) for 5 min, respectively, for further imaging using a confocal microscope (Olympus FV1000, Japan).
Food and Drug Administration; EpCAM, epithelial cell adhesion molecule; PCa, prostate cancer; EMT, epithelial-to-mesenchymal transition; PSA-EPISPOT, prostate-specific antigen-enzyme-linked immunospotassay; IV, intravenous; RBC, red blood cell; FACS, fluorescence-activated cell sorting; laPCa, locally advanced prostate cancer; DAPI, 4',6-diamidino-2-phenylindole.
The cells were incubated with VCAM-1 antibody (green) and nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI).
The nucleus was stained with 4',6-diamidino-2-phenylindole (DAPI).
Nuclei were visualized using 4',6-diamidino-2-phenylindole (DAPI) dihydrochloride (Sigma-Aldrich).
Slides were rinsed in PBS and counterstained with 4',6-diamidino-2-phenylindole (DAPI).